Question:
Did the fruit on the tree of knowledge of good and evil actually give them the knowledge of good and evil?

Answer:
God asked Adam: “Who told you that you were naked? Have you eaten from the tree of which I commanded you that you should not eat?” (Genesis 3:11).

It was the act of disobedience that gave them the knowledge of good and evil. Eve gained the knowledge that Satan promised her but she found out that it was a horrible knowledge. Satan tempted Eve with the lust of the flesh, lust of the eyes, and the pride of life (1 John 2:16). And it only took one temptation for Eve in the Garden of Eden to fall, but Jesus didn’t fall after being tempted with these same temptations for forty days without food in the wilderness. James 1:13-15 says that God is not responsible for the fall of man, but that man is.